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KMID : 1098420130210060466
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Korean Journal of Medicinal Crop Science
2013 Volume.21 No. 6 p.466 ~ p.473
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Effects of Supercritical Fluid Extract, Shikonin and Acetylshikonin from Lithospermum erythrorhizon on Chondrocytes and MIA-Induced Osteoarthritis in Rats
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Kim Geum-Soog
Kim Hwa-Jin
Lee Dae-Young
Choi Seung-Min
Lee Seung-Eun
Noh Hyung-Jun
Choi Jong-Gil
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Abstract
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This study investigates the effect of supercritical fluid extract (CMPB803-C) of Lithospermum erythrorhizon, shikonin and acetylshikonin isolated from Lithospermum erythrorhizon on IL-1β-induced chondrocytes and monosodium iodoacetate (MIA)-induced osteoarthritis in rat. Shikonin (50 μM) and acetylshikonin (3 μM) treatment reduced significantly the mRNA expression and enzyme activity of matrix metalloproteinase (MMP)-1, ?3 and ?13 in IL-1β-induced SW1353 chondrosarcoma cells. The chondro-protective effects of CMPB803-C and acetylshikonin were than analyzed in a rat OA model using a single intra-articular injection of MIA (1 ㎎) in the right knee joint. CMPB803-C (200 ㎎/㎏) or acetylshikonin
(5 ㎎/㎏) was orally administered daily for two weeks starting after 1 week of MIA injection. In the histological observation, CMPB803-C and acetylshikonin clearly improved OA lesions being comparable to or better that control group. Our results demonstrated that CMPB803-C and acetylshikonin as active compound of Lithospermum erythrorhizon have a strong chondro-protective effect in OA rats, which likely attributes to its anti-inflammatory activity and inhibition of MMPs production.
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KEYWORD
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Lithospermum erythrorhizon, Shikonin, Acetylshikonin, Osteoarthritis, MIA, MMP
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